engleski

Analysis of clonality in T-lymphoproliferative diseases by multiplex PCR

Distinction between benign and malignant T-cell lymphoproliferative disease can be difficult using morphological criteria. Using multiplex PCR system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T- lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal PCR products. By splitting the multiplex primer mix the patient specific TCRgamma rearrangement was determined: two of ten patients showed the exclusive presence of a single TCRgamma gene rearrangement. Three patients exhibited two rearranged TCRgamma genes, while in five patients positive reactions were obtained with three VJ primer combinations. Molecular analysis of rearranged T-cell receptor genes by multiplex PCR represents a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

T-cell receptor gamma gene rearrangement ; monoclonality ; lineage ; polymerase chain reaction

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