engleski

Decidual CD1a+ cells are able to shape the percentage and functions of CD56+bright and CD3+ cells at the maternal-fetal interface

Problem: Characteristics of decidual CD1a+ cells and their functional interactions with decidual lymphocyte subpopulations were investigated. Material and methods: The first trimester normal human pregnancy decidua was used for immunohistochemistry and the isolation of decidual mononuclear cells (DMC) by enzimatic digestion and gradient density centrifugation. Surface markers, intracellular cytokine and cytolytic mediators' expression in CD1a+ cells were analysed by multicolour flow cytometry. Decidual CD1a+ cells were isolated from the adherent, whereas CD56+ and CD3+ cells from the non-adherent DMC fraction by magnetic separation. They were co-cultured at different CD1a/NKcell and CD1a/T cell ratios, respectively. The proliferation, cytokines', cytolityc mediators' and NK cell receptors' expression were analyzed in CD56+ bright, as well as cytokine production in decidual T cells after the stimulation with CD1a+ cells. The cytotoxicity of CD1a+ cells against decidual T cells was tested using PKH-26 (red) cytotoxicity assay. Results: Decidua is abundant with CD1a+ cells that are mostly of myeloid origin with relatively low CD83, CD80, CD86 and CD56+ expression, but high HLA class I, HLA-DR and CD14 molecule expression. CD1a+ cells from freshly isolated suspensions express cytoloytic mediators and IL-15, IL-18, IFN- + and TNF- cytokines in the cytoplasm. CD1a+ cells enhance short and long term cytolytic mediators expression (18 hrs) and proliferation rate of NK cells (48 hrs), but decrease their pro-inflammatory (IL-15, IFN- TNF-α ) cytokines production and NKp46 receptor expression (18 hrs). Decidual CD1a+ cells by themselves neither affect IL- 4, nor IFN-g production in decidual T cells at DC/T cell ratio 1:5 after 3 days co-culture in vitro and kill CD3+ cells on a dose dependent manner. Conclusion: It seems that CD1a+ cells are able to shape the percentage and functions of CD56+bright and CD3+ cells at the maternal-fetal interface. Acknowledgement: The experiments were financed by the grants of &laquo ; ; ; EMBIC&raquo ; ; ; European FP6 project No. 512040, LSHM-CT-2004-512040 and Croatian Ministry of Science, Education and Sports Grants No. 0620402-0376 and No. 0377.

decidual mononuclear cells; decidua; pregnancy

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