engleski

Analysis of clonality in T-lymphoprolifertive diseases by multiplex PCR

Distinction between benign and malignant T-cell lymphoproliferative diseases can be difficult using morphological criteria. Using multiplex polymerase chain reaction system we have tested a series of patients with various lymphoproliferative disorders to detect clonal T-lymphocyte populations. Results show that clonal amplification products were obtained from all 10 patients with T-cell lymphoproliferative disorders while the amplification of DNA samples from B-cell neoplasms and normal individuals revealed polyclonal amplification products. By splitting the multiplex primer mix the patient specific T-cell receptor gamma-rearrangement was determined: five of ten patients showed the exclusive presence of a single T-cell receptor gamma-gene rearrangement. Three patients exhibited two rearranged TCR-gamma genes, while in two patients positive reactions were obtained with three pairs of primers for variable and joining segment. Molecular analysis of rearranged T-cell receptor genes by multiplex polymerase chain reaction represnets a useful and rapid tool for confirming diagnosis, to determine the extent of disease and to monitor the response to therapy.

PCR; TCR-gamma; acute leukemia; diagnosis

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