engleski

Does long-term exercise modulate oxidant/antioxidant status in humans? Comparison between lipid peroxidation and catalase activity in fresh and stored samples

Physical activity may have beneficial effects on the balance between oxidant stress and the defense system. The purpose of this study was to evaluate the influence of exercise on oxidant/antioxidant status in both sexes during autumn and winter periods. Since all women were tested in a low estrogen phase, the possibility to test the same parameters in plasma stored at low temperature would be of great importance. Oxidant changes in plasma were determined by measuring the concentration of thiobarbituric acid reactive substances (TBARS). Antioxidant capacity was measured by catalase (CAT) activity in lysate of erythrocytes. The results showed significantly higher TBARS concentrations in untrained men compared to women. Antioxidant capacity was of the same value in both sexes. Exercise did not change the oxidant/antioxidant status of either sex. The results indicate that women were more protected from oxidant processes than men. This finding points to the role of lipid peroxidation in higher incidence of myocardial infarction in men during winter. Significant changes in the oxidant/antioxidant status in stored plasma allowed greater reliability of TBARS and CAT measurements only in fresh samples.

lipid peroxidation; catalase; exercise; oxidant; antioxidant

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