engleski

Morphohistochemical profile of red and pink muscles in freshwater fish

The morphohistochemical profile of red and pink muscles from the caudal portion of the trunk at the heightof the horizontal septum in the Carassius auratus gibelio and Perca fluviatilis 20-25 cm long from the Garešnica (Croatia) fish farm have been studied. Muscle samples, taken on arrival from live fish, were frozen in liquid nitrogen, cut on the cryo cut into 10 µ ; ; m thick slices stained with hemalum and eosin and with the procedure for the detection of succinate dehydrogenase (SDH). The diameter of muscle fibers from the obtained preparations was measured using a micrometer scale with 10 µ ; ; m divisions on a Nikon microscope. The red muscle of the Carassius auratus gibelio and Perca fluviatilis is known to have a triangular cut. It is positioned laterally beneath the skin and runs craniocaudally along the horizontal septum. Underneath the surface red muscle there is a pink muscle, shorter than the red one. In the Carassius auratus gibelio it is wide and divided into the surface (pink I) and deep (pink II) layer, while in Perca fluviatilis it is narrow, short and unchanged, but comprises a narrow transitional zone towards the red muscle. The red muscle is composed ofred muscle fibers of small diameter with potent SDH activity. The pink muscle is composed of two types of fibers . intermediary small-diameter muscles of pronounced SDH activity and large-diameter muscles of less pronounced SDH activity as well as white muscles of large diameter with poor SDH activity. Intermediary fibers in the Carassius auratus gibelio accounted for 77.80% of the fibers in the pink I layer and 44.90% of the fibers in the pink II layer. White fibers in the same fish accounted for 22.20% of the fibers in the pink I layer and 55.10% of the fibers in the pink II layer. Intermediary fibers in the pink muscle of the Perca fluviatilis accounted for 58.80% fibers, whereas white fibers accounted for 41.20% of all fibers.

red muscle; pink muscle; Carassius auratus gibelio; Perca fluviatilis; morphohistochemical

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