engleski

Phenotypic Analysis of Lymphatic Organs in CD26 Deficient Mice

The surface antigen CD26 (dipeptidyl peptidase IV, DPP IV/CD26, EC 3.4.14.5.) is a multifunctional transmembrane glycoprotein which has been shown to play an important role in T cell activation and functions of the immune system. The aim of this study was to elucidate the characteristics of phenotypic profiles of hepatic and splenic mononuclear lymphatic cells (MNLC) and thymocites of CD26 gene knockout mice with C57BL/6 background in comparison with wild type C57BL/6 mice. The changes in the percentage of CD3+CD69+NK+, CD3+CD122+, CD4+CD8+ and CD25+CD4+ hepatic and splenic MNLC and thymocites were identified by flow-cytometric analyse (FACSCalibur, Becton Dickinson). Data were analyzed using the Sigma Plot Scientific Graphing System, Version 6.10. The statistical significances were evaluated using Mann-Whitney U-test. The differences were considered significant for p<0.05. The results of our study show that CD26 deficient mice have a statistically significant increase in the proportion of hepatic mononuclear cells ; NK, NKT cells (both phenotypes: 3+NK+ and 3+122+) and CD122 (IL2Rβ ). A significant augmentation in the values of CD3, NKT cells and double positive CD4+CD8+ cells was also noticed in the spleen. Although CD26 deficient mice apparently display a normal phenotype, our data indicate that they differ from their genetic background, wild type C57BL/6. This leads to the conclusion that the CD26 molecule could be involved in the regulation of immune parameters, which makes it important for the development and progression of immune mediated diseases.

Phenotypic analysis; CD26 deficient mice

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