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Effect of propolis and its olyphenolic/flavonoids compounds on dna damage induced by radiation to mouse lymphocytes

This study assessed the antioxidant potencies of several widespread flavonoids present in propolis and proplis alone. CBA mice were injected intraperitoneally (ip) with two preparation of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and propolis polyphenolic compounds (caffeic acid, naringin, chrysin, quercetin) at dose of 100 mg kg-1 body weight for 3 conecutive days before or after whole body γ -irradiation (WBI). Synthetic protector 2-aminoethylisothiouronium bromide hydrobromide (AET) was used as a positiv control. Mice were exposed to WBI with 9 Gy of 60Co γ -radiation source. Therty min. after iradiation and/or treatment with test components we examined DNA damage of lymphocytes using the single-cell gel electophoresis assay (comet assay). The WBI of mice resulted in a significant elevation of DNA damage of lymphocytes as compared with unradiated mice.Pretreatment of mice with WSDP or EEP and flavonoids produced the reduction in oxidative DNA damage of lymphocytes as compared with control and they were ranked in decreasing order of potency as follows: naringin (2.98%) ; chrysin (16.84%) ; quercetin (33.67%) ; AET (48.52%) ; caffeic acid (49.51%) ; EEP (53.47%) ; and WSDP (54.46%), respectively. Also treatment with propolis and its polyphenolic/flavonoids compounds after irradiation resulted in a significant reduction of DNA damage as follows: caffeic acid (32.31%) ; AET (75.39%) ; naringin (78.46%) ; EEP (80%) ; chrysin (83.08%) ; quercetin (84.62%) ; and WSDP (89.24%). These data suggest that WSDP and EEP are more protective than flavonoids from propolis alone. Data are also consistent with the hypothesis that radioprotective activity of EEP and/or WSDP related to synergistic antioxidative effect of components present in EEP or WSDP.

propolis; polyphenolic; lymphocytes

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