engleski

Cellular uptake quantification of metallated peptide and peptide nucleic acid bioconjugates by atomic absorption spectroscopy

This work investigates the use of peptides and peptide nucleic acid oligomers (PNA) as vectors for the cellular uptake and nuclear delivery of metal complexes. Atomic absorption spectroscopy (AAS) is used as the metal-specific detection method. Compared to previous studies, which use indirect methods such as fluorescence spectroscopy or splice-correction assays, the metal-specific AAS allows accurate determination of intracellular concentrations of the conjugates. It is thus most suited to establish uptake efficiencies for any metal-containing bioconjugate and is applied herein to study the cell uptake of PNA oligomers and peptides for the first time. For the best systems in this study, an accumulation 150% higher than in the cell culture medium was achieved.

cobalt ; medicinal chemistry ; N ligands ; nucleic acids ; peptides

nije evidentirano