Glycation of a lysine-containing tetrapeptide by D-glucose and D-fructose - Influence of different reaction conditions on the formation of Amadori/Heyns products (CROSBI ID 137334)
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Jakas, Andreja ; Katić, Anja ; Bionda, Nina ; Horvat, Štefica
engleski
Glycation of a lysine-containing tetrapeptide by D-glucose and D-fructose - Influence of different reaction conditions on the formation of Amadori/Heyns products
The site specificity, extent and nature of modification of the tetrapeptide, Leu-Ser-Lys-Leu (1), incubated with D-glucose or D-fructose in methanol, or in phosphate buffer of pH 5.7, 7.4 and 8.0 were investigated. The generated mono- and di-glycated Amadori (1-deoxy-D-fructosyl derivatives) and Heyns rearrangement products (N-alkylated glucosamine/mannosamine derivatives) were isolated and characterized by NMR and mass spectrometry. The results identified the  -amino group of the Lys residue as the preferential glycation site in tetrapeptide 1. Under all conditions investigated, glucose afforded higher yields of glycation products than fructose. In the reactions carried out in buffer, glycation at pH 7.4 and 8.0 was much faster than at pH 5.7.
Amadori compound; Fructose; Glucose; Glycation; Heyns compound; Maillard reaction
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