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izvor podataka: crosbi

Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae) : intraspecific variation and differentiation from Fasciola hepatica (CROSBI ID 137491)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Kralova-Hromadova, Ivica ; Špakulova, Marta ; Horačkova, Eva ; Turčekova, Ludmila ; Novobilsky, Adam ; Beck, Relja ; Koudela, Bretislav ; Marinculić, Albert ; Rajsky, Dušan ; Pybus, Margo Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae) : intraspecific variation and differentiation from Fasciola hepatica // The Journal of parasitology, 94 (2008), 1; 58-67

Podaci o odgovornosti

Kralova-Hromadova, Ivica ; Špakulova, Marta ; Horačkova, Eva ; Turčekova, Ludmila ; Novobilsky, Adam ; Beck, Relja ; Koudela, Bretislav ; Marinculić, Albert ; Rajsky, Dušan ; Pybus, Margo

engleski

Sequence analysis of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna (Trematoda: Fasciolidae) : intraspecific variation and differentiation from Fasciola hepatica

Complete sequences of ribosomal and mitochondrial genes of the giant liver fluke Fascioloides magna are presented. In particular, small subunit (18S) and internal transcribed spacers (ITS1 and ITS2) of the ribosomal gene (rDNA), as well as cytochrome c oxidase subunit I (cox1) and nicotinamide dehydrogenase subunit I (nad1) of the mitochondrial DNA (mtDNA), were analyzed. The 18S and ITS sequences were compared with previously published sequences of the liver fluke Fasciola hepatica. Fixed interspecific genetic differences were determined that allow molecular differentiation of F. magna and F. hepatica using either the PCR-RFLP method or PCR amplification of species-specific DNA regions. Additionally, intraspecific sequence polymorphism of the complete cox1 and nad1 mitochondrial genes in geographically distinct F. magna populations was determined. Based on the sequence divergences, short (500 bp) variable regions suitable for broader biogeographical studies of giant liver fluke were designed.

Fascioloides magna; F. hepatica; intraspecific variation; differentiation; sequence analysis

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Podaci o izdanju

94 (1)

2008.

58-67

objavljeno

0022-3395

Povezanost rada

Veterinarska medicina

Indeksiranost