engleski

First record of Pech latent mosaic viroid in Croatia

Throughout the years 2003 and 2004, 168 Prunus trees were analyzed by tissue-printing molecular hybridization for the presence of Peach latent mosaic viroid (PLMVd) and Hop stunt viroid (HSVd). This survey was carried out in different Croatian continental and coastal (Mediterranean) stone fruit growing regions. The sampling encompassed various cultivated stone fruit species (peach, nectarine, apricot, sweet and sour cherry), wild Prunus (P. myrabolana, P. mahaleb, P. padus, P. spinosa) as well as urban ornamentals (P. juliana, P. laurocerasus, P. serrulata, P. subhirtela and P. virginiana). The trees were located in commercial orchards, stone fruit collections, family gardens, or they were sampled as individual urban trees. Collection of ornamental Prunus from the Botanical Garden of the Department of Biology in Zagreb was also included in this survey. PLMVd was detected only in nectarine and peach trees with the incidence of 94% and 42%, respectively. Infected nectarines, originating from Italy, were grown in both climatic regions. PLMVd infected peaches originated both from imported and native cultivars. The PLMVd-positive samples obtained by tissue printing hybridization were confirmed by RT-PCR. The HSVd presence in the surveyed Prunus trees has not been unequivocally proved. None of the randomly tested wild Prunus or the ornamental trees has resulted viroid-positive. This is the first molecular probing of PLMVd and HSVd in Croatia. Nonetheless its preliminary nature, it can help in developing comprehensive testing schemes and strategies for stone fruit tree viroid control.

PLMVd; nectarine; peach; tissue-printing; RT-PCR

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