Human dipeptidyl peptidase III acts like a post-proline-cleaving enzyme on endomorphins (CROSBI ID 137602)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Baršun, Marina ; Jajčanin, Nina ; Vukelić, Bojana ; Špoljarić, Jasminka ; Abramić, Marija
engleski
Human dipeptidyl peptidase III acts like a post-proline-cleaving enzyme on endomorphins
The dipeptidyl peptidase III (DPP III) is a zinc-exopeptidase with a role indicated in mammalian pain-modulatory system, due to its high affinity for enkephalins and localization in the superficial laminae of the spinal cord dorsal horn. Our study revealed that this human enzyme hydrolyses opioid peptides belonging to the three new groups, endomorphins, hemorphins and exorphins. The enzymatic hydrolysis products of endomorphin-1 were separated and quantified by capillary electrophoresis and kinetic parameters determined for human DPP III and rat DPP IV. Both peptidases cleaved endomorphin-1 with comparable rate, by liberating the N-terminal Tyr-Pro. This is the first evidence on DPP III acting as a endomorphin-cleaving enzyme.
Aminopeptidase ; Capillary electrophoresis ; Hydrolase ; Opioid peptides
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