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Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment (CROSBI ID 137682)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Šegvić Klarić, Maja ; Rumora, Lada ; Ljubanović, Danica ; Pepeljnjak, Stjepan Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment // Archives of toxicology. Supplement, 82 (2008), 4; 247-255

Podaci o odgovornosti

Šegvić Klarić, Maja ; Rumora, Lada ; Ljubanović, Danica ; Pepeljnjak, Stjepan

engleski

Cytotoxicity and apoptosis induced by fumonisin B1, beauvericin and ochratoxin A in porcine kidney PK15 cells: effects of individual and combined treatment

The objective of this study was to determine individual and combined effects of fumonisin B(1) (FB(1)), beauvericin (BEA) and ochratoxin A (OTA) on porcine kidney epithelial PK15 cell survival by measuring lactate dehydrogenase (LDH) activity, apoptotic index and caspase-3 activity. Cells were treated with 0.05, 0.5 and 5 mug/ml of each mycotoxin or with the combinations of two or all three mycotoxins for 24 and 48 h. Changes in LDH and caspase-3 activity, and in apoptotic index showed that the cytotoxic and apoptotic effects of these mycotoxins were concentration- and time- dependent. Significant increase of LDH activity was observed after 48 h of exposure to the highest concentration of FB(1) (45%), BEA (84%) and OTA (77%), as compared to control. OTA increased caspase-3 activity after 24 h of treatment with 0.5 mug/mL (84%), while BEA (319%) and FB(1) (419%) significantly affected this enzyme activity after 48 h (P < 0.05). Increase of caspase-3 activity preceded significant morphological apoptotic changes, which were detected after 48 h of exposure to a single toxin. Combined treatment with FB(1), BEA and OTA resulted mostly in additive effects on LDH activity, and additive and synergistic effects on caspase-3 activity and apoptotic index.

Fumonisin B1; Beauvericin; Ochratoxin A; Necrosis; Apoptosis; Caspase-3; Mycotoxin interactions

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Podaci o izdanju

82 (4)

2008.

247-255

objavljeno

0171-9750

Povezanost rada

Temeljne medicinske znanosti, Farmacija