engleski

Molecular markers for QTL detection in segregating maize populations derived from exotic germplasm

The major objectives of this study included: 1) detect QTLs for grain yield (YLD), plant height (PH), and days to pollen shed (DPS) in maize exotic germplasm, and 2) to determine the relationship between the degree of heterozygosity of marker alleles and the level of yield. Lines selected from Croatian open-pollinated varieties were used to generate two half-sib populations for the study. In each population, 163 F-3 families were tested in field experiments at two locations. The 20 highest and the 20 lowest yielding families of each population were used for QTL detection. Isozymes, RFLPs and Simple Sequence Repeats (SSRs) were used as molecular markers. Marker-trait associations were assessed by single factor and interval analyses. The population designated P-1 had lower average yield but had more polymorphic loci and revealed more QTLs than the population designated P-2. In the two populations, QTLs for YLD were found on chromosomes 1, 6, and 10; for PH on chromosomes 1, 3, 5, and 6; and for DPS on chromosomes 1, 3, 6, 8 and 9. The populations had no QTLs in common. Higher yielding subpopulations showed a higher degree of heterozygosity than the lower yielding subpopulations. [References: 32]

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