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Murine cytomegalovirus clear MHC class I molecules from the cell surface of infected cell and retrieve them in the juxtanuclear pre-late endosomal compartment (CROSBI ID 535305)

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Ilić Tomaš, Maja ; Kučić ; Natalia ; Mahmutefendić, Hana ; Blagojević, Gordana ; Lučin, Pero Murine cytomegalovirus clear MHC class I molecules from the cell surface of infected cell and retrieve them in the juxtanuclear pre-late endosomal compartment // American journal of reproductive immunology / Kenneth Beaman, Daniel Rukavina (ur.). 2007. str. 430-430

Podaci o odgovornosti

Ilić Tomaš, Maja ; Kučić ; Natalia ; Mahmutefendić, Hana ; Blagojević, Gordana ; Lučin, Pero

engleski

Murine cytomegalovirus clear MHC class I molecules from the cell surface of infected cell and retrieve them in the juxtanuclear pre-late endosomal compartment

Problem. Murine cytomegalovirus (MCMV) downregulate MHC class I molecules (MHC-I) from the cell surface at early stages of infection. The aim of our study was to compare intracellular localization of MHC-I on uninfected and MCMV-infected mouse embrional fibroblasts (MEF). Methods of study. MEFs were infected with deletion mutant of MCMV devoid of viral Fc receptor gene, and intracellular localization and trafficking of MHC-I molecules was determined by confocal microscopy. Cell surface expression of MHC-I molecules was followed by flow-cytometry and intracellular stability by immunoprecipitation after cell surface biotynilation. MHC-I were labeled with monoclonal antibody MA-215 (Kd) and 34-5-8s (Dd). In order to define a compartment wherein MHC-I are accumulated colocalization with a Golgi marker (GM130), an early endosome marker (EEA-1), a lysosomal marker (LAMP-1) and with antibody against TfR (R-17) were used. Microtubular network was disrupted by nocodazole (6.6 μ M). Results. We have found that Kd and Dd molecules, which are present at the surface of uninfected cells, are down-regulated from the surface of infected cells. These molecules can be found accumulated in juxtanuclear region of infected cells in large vesiculo-tubular structures. Kd and Dd molecules colocalized with GM130, R-17 and in small amount with LAMP-1 and EEA-1. In order to dissect whether these molecules are retrieved by the Golgi or in endosomal vesicles, we used nocodazole to disperse juxtanuclear organelle, including the Golgi complex. After nocodazole treatment, Golgi complex was dispersed into vesicles containing Kd and Dd molecules, however almost half of retained molecules did not colocalize with GM130. Conclusions. From these results we can conclude that virus down-regulates the MHC-I from the cell surface of infected cells into the juxtanuclear vesicles that are after sorting endosomes in the endocytic pathway (EEA-1) and before late endosomes (Lamp1). These vesicles are outside of the recycling route (TfR-). Significant amount of the nascent MHC-I molecules are, in addition, retained in the Golgi complex. Supported by Croatian Ministry of Science, Grant No 0620238-0223.

Murine cytomegalovirus; MHC class I molecules

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Podaci o prilogu

430-430.

2007.

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objavljeno

Podaci o matičnoj publikaciji

American journal of reproductive immunology

Kenneth Beaman, Daniel Rukavina

Oxford: Blackwell Publishing

1046-7408

Podaci o skupu

X International Congress of rEproductive Immunology

poster

10.06.2007-14.06.2007

Opatija, Hrvatska

Povezanost rada

Temeljne medicinske znanosti

Indeksiranost