hrvatski

Utjecaj pasmine, pariteta i laktacije na koncentraciju PAG i progesterona tijekom gravidnosti Sanskih i Burskih koza

The aim of the study was to investigate role of breed, parity and lactation on PAG and progesterone profiles in pregnant saanen and boer goats. All saanen goats were in lactation and milked twice daily till the last trimester of pregnancy. All boer goats were separated from previous kidds and had no milk production. We had two herds of goats, boer goats (n=25) and saanen goats (n=22). We subdivided herds in 2 subgroups each: pluriparous pregnant (boer goats n=17, saanen goats n=14) and primiparous pregnant goats (boer goats n=8, saanen goats n=8). We monitored natural mating of all goats during november 2006. We monitored pregnancy 35 days after mating and monthly later to follow fetal development and detect embrionic mortality using ultrasound probe. The fetuses were identified, counted by ultrasound and later the numbers were compared with the kidding rate. Only goats who kidded at the end of the study were taken in final consideration and used in study. Blood samples (10 ml) were collected from the jugular vein in heparinized vacutainer tubes at weeks 0, 1, 2, 3, 4, 6, 8, 10, 12, 14, 16, 18, and 20 after mating and on the day of parturition. Plasma was removed by centrifugation (at 1500 g for 15 min) immediately after collection and stored at -20°C until assayed for PAG and progesterone. Level of progesterone and PAG in sera samples was determined using standard RIA method. PAG was determined by method described by Zoli and modified by Beckers. Progesterone was determined using standard procedure with conjugated steroids (Coat-A-Count TKPG, Diagnostic Product Corporation) without preincubation. The 125I in the pellet was quantified using gamma counter (LKB Wallac 1261 Multigamma counter, LKB, Turku, Finland) with a counting efficiency of 75%. All samples were analysed in duplicate with middle value like result and all suspect results were repeated. Program Statistica and ANOVA to perform variance analysis and linear correlations were used. PAG and progesterone level were compared between primiparous and pluriparous groups according to breed, parity and lactation differences. Progesterone and PAG level curve was significantly higher in pregnant pluriparous goats of both breeds comparing to summarized results for both primiparous group (p<0, 05). Number of kidds didn't differ between all four groups (2, 4 and 2, 6 for saanen groups versus 2, 6 and 2, 8 kidds for boer groups) and couldn't influence progesterone nor PAG level. Primiparous goats of both breeds had statistically significant higher individual variations (maximum and minimum) for both parameters (progesterone and PAG) during pregnancy (p<0, 05). We compared levels of progesterone and PAG from lactating saanen goats with pluriparous nonlactating boer goats in the same stage of pregnancy and didn't find any significant difference. We concluded that parity influences both level of progesterone and PAG in pregnant goats of both breeds but lactation and breed had no significance.

graviditet; koza; paritet; laktacija; progesteron; PAG

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