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izvor podataka: crosbi

Differential sensitivity to 4-hydroxynonenal for normal and malignant mesenchymal cells (CROSBI ID 140000)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Borović, Suzana ; Čipak, Ana ; Meinitzer, Andreas ; Kejla, Zvonko ; Perović, Darko ; Waeg, Georg ; Žarković, Neven Differential sensitivity to 4-hydroxynonenal for normal and malignant mesenchymal cells // Redox report, 12 (2007), 1/2; 50-54. doi: 10.1179/135100007X162194

Podaci o odgovornosti

Borović, Suzana ; Čipak, Ana ; Meinitzer, Andreas ; Kejla, Zvonko ; Perović, Darko ; Waeg, Georg ; Žarković, Neven

engleski

Differential sensitivity to 4-hydroxynonenal for normal and malignant mesenchymal cells

Normal and malignant cells of various origin differ in their sensitivity to oxidative stress. Therefore, we used normal and malignant mesenchymal cells-human osteosarcoma cells (HOS and 143B), human fibroblasts (WI38) and two primary cultures of normal human osteoblasts to test sensitivity to reactive aldehyde 4- hydroxynonenal (HNE), known as a second messenger of free radicals and a signaling molecule. Upon HNE-treatment, decrease in cell viability (by Trypan-blue), apoptosis induction (by TiterTACS (TM) TUNEL assay), HNE-protein binding (by HNE-His ELISA) were higher in malignant than in normal cells, while glutathione content was higher in normal cells. These results indicate that HNE affects the growth of malignant mesenchymal cells more than normal and that this effect was mainly related to lower glutathione concentration and higher binding of HNE to the cellular proteins. We thus assume that HNE and GSH homeostasis play an important role in the growth regulation of normal and malignant mesenchymal cells.

4-hydroxynonenal; mesenchymal cells; human osteosarcoma; human osteoblasts; apoptosis; hne-his elisa; glutathione; persistent oxidative stress; human osteosarcoma cells; lipid-peroxidation; glutathione; conjugation; cancer; localization; products; adducts; tissues

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Podaci o izdanju

12 (1/2)

2007.

50-54

objavljeno

1351-0002

10.1179/135100007X162194

Povezanost rada

Temeljne medicinske znanosti

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