4-hydroxynonenal enhances permeability of in vitro intestinal barrier model (CROSBI ID 536543)
Prilog sa skupa u zborniku | sažetak izlaganja sa skupa
Podaci o odgovornosti
Borović Šunjić, Suzana ; Štroser, Marina ; Živković, Emilija ; Žarković, Neven
engleski
4-hydroxynonenal enhances permeability of in vitro intestinal barrier model
Human colon adenocarcinoma cell line CaCo-2 was shown to undergo spontaneous in vitro enterocytic differentiation leading to the formation of cell monolayer with several morphological an functional characteristics of mature enterocytes. Gastrointestinal tract is often exposed to harmful compounds originating from diet ; they can cause oxidative stress and intestinal dysfunction. Such dysfunction can make the intestinal barrier permeability greater for toxins, bacteries, harmful products of digestion, free radicals and second messangers of free radicals like 4-hydroxynonenal (HNE). HNE is one of major lipid peroxidation product, and because of its lipophilic caracter, it can be readily absorbed in the intestine or can accumulate in membranes causing phospholipid and protein damage. Cell monolayer was good developed after 15th day and represents intestinal barrier model. HNE impaired function of intestinal barrier model and caused increase in its permeability. With this study, we have shown that in vitro model of intestinal barrier could be used for oxidative stress research.
CaCo-2; differentiation; 4-hydroxynonenal; HNE; intestinal barrier; antioxidants; oxidative stress; gastrointestinal tract
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Podaci o prilogu
2007.
objavljeno
Podaci o matičnoj publikaciji
Abstracts of the COST Meeting "Oxygen, stress and lipids"
Podaci o skupu
COST Meeting "Oxygen, stress and lipids"
poster
26.10.2007-27.10.2007
Dubrovnik, Hrvatska