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Measurement of malondialdehyde (MDA) level in rat plasma after simvastatin treatment using two different analytical methods

The aim of study was to investigate the effect of chronic administration of simvastatin (SIMV) on plasma malondialdehyde (MDA) level using two different methods. We also wanted to examine the plasma MDA level 10 days after the last administration of SIMV. The first two groups of Wistar rats were given 10 mg/kg/day of SIMV and the third and fourth groups 50 mg/kg/day of SIMV for 21 days. Plasma MDA level was measured after the end of treatment and 10 days after the last dose. Two methods were used: UV-VIS spectrophotometric method and HPLC-MS method. Statistics: Kruskal-Wallis test and Steel test for post-hoc comparison with the control group. P values less or equal to 0.05 were considered as statistically significant.According to the results, MDA levels in all groups, meaured by both techniques, showed that SIMV treatment caused a dose-dependent decrease (significant in high dose) in plasma MDA level. The decrease in MDA level was also well-maintained for 10 days after the last administration of SIMV in both doses (Significantly in high dose. These results showed that SIMV has antioxidant activity that persists after discontinuation of therapy.

statins; simvastatin; malondialdehyde; lipid peroxidation; HPLC-MS; spectrophotometry; oxidative stress

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