engleski

Survival of rat and mouse foetuses after treatment with teratogens

Mouse and rat experimental animals are the most common mammalian models for teratogenic investigation. In our previous work we investigated the teratogenic effect of 5-azacytidine (5azaC) on rat limb development, and discovered the protective effect of aspirin (ASA), although it is known that aspirin given in high doses also acts as a teratogen. In this work, we investigated the influence of these teratogens combination on mouse and rat foetal survival. The goal of these experiments was to determine whether there is any difference in susceptibility to these teratogens between the mouse and the rat. 5azaC was dissolved in PBS and injected intraperitonealy at a concentration of 5 mg/kg body weight to pregnant rat and mouse females on 13th and 11th day of pregnancy respectively. ASA was dissolved in PBS and administered 2 hr before 5azaC at a concentration of 75 mg/kg. Controls were treated with PBS on the same gestation days. Animals were killed the day before birth. Foetal status (survival, intrauterine death and resorption) was determined. In mouse model 5azaC caused 30% i.u. deaths, no resorptions at all while combination of 5azaC, and presumably, protection agent ASA caused almost 27% i.u. deaths and even 15% resorptions. On the other hand, in rat model neither 5azaC nor 5azaC and ASA combination caused any i.u. deaths or resorptions. Controls of both models showed 100% foetal survival. In conclusion, the mouse model seems to be more susceptible than rat to the influence of investigated teratogens.

rat; mouse; 5-azacytidine; ASA

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