engleski

Structural investigation of new insulin derivative at room temperature

Insulin is a hormone protein that regulates carbohydrate metabolism and it also takes part in the metabolism of fat and proteins. It is used medically in patients with Type 1 diabetes mellitus. Occasionally some patients with Type 2 diabetes mellitus also require insulin. Owing to its crucial metabolic role and its pharmaceutical importance many structural studies on chemically and genetically modified insulins have been done. We will present the results of our investigation on human bromo- derivative of insulin. Both single crystal and powder diffraction data were collected on laboratory instruments at room temperature. The investigated insulin derivative belongs to the T3R3f rhombohedral form [1] with cell parameters a = 80.96 Å ; ; ; ; and c = 37.30 Å ; ; ; ; . The unit cell parameter c at room temperature is two times smaller in comparison to the one at 100 K [2]. There is one zinc ion on the three fold axis and the second one in the general site surrounding the threefold axis. Both zinc ions are tetrahedrally coordinated.

insulin; protein crystallography; powder diffraction

doi: 10.1107/S0108767307095693