engleski

Cytotoxicity of propolis and its polyphenolic compounds on primary culture of human urinary bladder transitional cell carcinoma

This study was carried out to find whether propolis and its polyphenolic/flavonoids compounds may induce cytotoxicity in primary culture of human urinary bladder transitional cell carcinoma (TCC) cells as compared to normal urinary bladder epithelial cells. Pieces of TCC or normal epithelial tissue were collected by transurethral surgery from patients in different stages (grade G1, G2, G3) of TCC. Incubation of TCC cells for cytotoxicity testing were carried out with or without diffrent concentration (50, 150, 300 µ ; g/ml) of test components. The cytotoxicity of two preparation of propolis (water and ethanolic extract of propolis ; WSDP or EEP) and its polyphenolic compounds (caffeic acid, naringin, chrysin, quercetin) was determined using trypan blue exlusion assay. Findings suggest that EEP is the most effective in inhibition of urinary bladder TCC cell proliferation as compared to WSDP or single flavonoids derived from propolis. All test components showed no cytotoxicity to normal epithelial cells. The result of this study may provide great impact on the potential activity of EEP as an adjuvant to surgery, to suppress or prevent tumor recurrence in urinary bladder since only a few anti-cancer drugs have been effective in tumor control. Since immunomodulation by BCG has been used to impruve the results of surgery it is likely that propolis preparation (EEP) as immunomodulating compound may be a substitute for mycobacterial treatment since propolis preparation or its polyphenolic components have expressed no side effect after treatment in animal models.

propolis; flavonoids; human urinary bladder cancer; cytotoxicity; primary culture

Rad je kao poster prezentiran na skupu "3rd International Conference on Polyphenols and Health", održanom od 25-28.11.2007., Kyoto, Japan ; i kao pozvano predavanje na skupu "Apimedica 2006 : 1st International Forum on Apitherapy", održanom od 12-15.10.2006., Atena, Grčka.