engleski

Patulin production by Penicillium expansum ZMPBF 565 in apple juice

The aim of this study was to investigate patulin biosynthesis during the growth of mould Penicillium expansum ZMPBF 565 in liquid media (apple juice). Experiments were carried out in a stationary culture and on shaker at incubatuion temperature of 18oC for 9, 12 and 15 days. At each time point mycelial dry weight were determined gravimetrically in a liquid media and the concentrations of patulin was measured fluorodensimetrically using Camag TLC scanner. The greatest amount of biomass was obtained in experiments on shaker, but the concentration of patulin was lowered. In stationary culture the highest increase in biomass growth was found after 15 days. The patulin accumulation started 9 days after inoculation, and the maximum concentration was reached 15 days. During the growth of mould P. expansum ZMPBF 565 on shaker the biomass synthesis was increased, but the patulin syntzhesis decreased. The maximal biomass obtained on shaker was reached 9 days after inoculation, while the highest concentration of patulin was found after 15 days.

Patulin; Penicillium expansum; biosynthesis; apple juice

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