engleski

Incidence of human papillomavirus infection in population of women from eastern Croatia

Today, more then 130 types of human papillomavirus (HPV) are known, of which about 40 induces genital infections. High-risk types (HR-HPV) are frequently associated with development of cervical intraepithelial neoplasia (CIS), carcinoma in situ and cervical cancer, while low-risk types (LR-HPV) are mainly associated with genital warts. Following Papa test and HPV genotyping we have attempted to determine frequency and distribution of HR- and LR-HPV in frequently diagnosed disorders ; CIN-I, CIN-II, CIN-III, condyloma accuminata and portio suspect. DNA was isolated from cervical samples of 523 women and β -globin PCR was used for DNA quality control. PCR was inhibited in 21 (4.01%) samples. Detection and genotyping of L1 virus gene was performed by PCR-ELISA method, using consensus primers (MY09, MY11) and biotin-labeled hybridization probes specific for different HPV types. L1 negative DNA samples were additionally tested using E6/E7 consensus primers. Statistical analysis was performed by Pearson's χ 2 or Fisher exact test. HPV was determined in 228 (45.32%) specimens. Significantly higher frequency of HR-HPV genotypes was observed in CIN-II group of samples (χ 2=4.835, p=0.0286 ; OR=1.82 (95%CI 1.02-3.21)). After results were reorganized in two groups, women over and under 30 years of age, higher frequency of LR-(χ 2=5.885, p=0.015 ; OR=2.18 (95%CI 1.11-4.44)) and HR-HPV (χ 2=6.390, p=0.011 ; OR=1.88 (95%CI 1.12-3.20)) was observed in CIN-I group of younger women. Even though, our results indicate stronger association between HR-HPV and higher grades of CINs, transforming potential of LR-HPV can’ t be ignored due to their high prevalence in CIN- I group of younger women.

human papillomavirus; women; Eastern Croatia; cervical cancer

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