engleski

Cythopathic Structuree Associated with Robinia Mosaic Virus, a Strain of Peanut Stunt Virus

Leaf cells infected with robinia mosaic virus from particular areas with well-formed symptomes have been investigated by light and electron microscopy for intracellular changes. Light microscopy of living tissue of Chenopodium quinoa and Nicotiana megalosiphon showed large crystalline inclusion in the form of different polyhedral bodies in some epidermal and mesophyll cells. These inclusions resambled the large virus crystals with hollow centres often found with the central vacuole in cuccumber mosaic virus (CMV) infections. The form of the polyhedral crystals and their frequency were influenced by external conditions and not by the host plant as previously recorded. The sequence of setting of the virus in formation of polyhedral crystals is discussed. In thin sectioons of infected C. quinoa accumulations of small crystals built of doughnut-like virus particles were seen in the vacuole. Small vesicles associated with the tonoplast, appearing to be a general characteristic of cucumoviruses, were often observed. Some other cytopathic structures typical of CMV were also found. darkly stained rough endoplasmic reticulum suggested specific activity in infected cells.

Robinia Mosaic Virus; light and electron microscopy; crystals; cytopathic structures

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