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Light Microscopy of Robinia Mosaic cucumovirus Crystalline Inclusions

In cells infected with robina mosaic cucumovirus (RoMV) we estabished two basic types of crystalline virus inclusions: hollow crystals, of polyhedral and spherical to oval appearance, and ordinary polyhedral crystals. The former seem regularly present in the vacuole, and the latter in the cytoplasm. The optimal temperatures for development of RoMV crystalline inclusions were betwenn 15-20 C at the period of longer days. All forms of the crystals were more frequent in relatively younger cells in which more intense filling of the hollow crystals took place.

Robinia Mosaic Virus; light and electron microscopy; crystals; cytopathic structures

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