The role of antibodies specific for toxic sPLA2s and haemorrhagins in neutralizing potential of antisera raised against Vipera ammodytes ammodytes venom (CROSBI ID 144159)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Halassy, Beata ; Habjanec, Lidija ; Brgles, Marija ; Lang Balija, Maja ; Leonardi, Adrijana ; Kovačič, Lidija ; Prijatelj, Petra ; Tomašić, Jelka ; Križaj, Igor
engleski
The role of antibodies specific for toxic sPLA2s and haemorrhagins in neutralizing potential of antisera raised against Vipera ammodytes ammodytes venom
The contribution of antibodies directed against the two main toxic groups of proteins in the Vipera ammodytes ammodytes venom, haemorrhagic metalloproteinases (H) and neurotoxic sPLA2s (Atxs), to the overall protective efficacy of the whole venom antisera was investigated. Using ELISA assays we established a high correlation between the protective efficacy of the whole venom antisera in mice and their anti-Atxs antibody content. As the haemorrhage is the prevailing toxic effect of the venom in human, the lack of correlation also with anti-H IgG content exposed that the mouse model might not be optimal to evaluate the neutralizing potential of the venom-specific antisera for human therapy. We further revealed that Atxs and structurally very similar but non-toxic AtnI2 from the venom are not immuno cross-reactive.
Vipera ammodytes ammodytes; Phospholipase A2; Ammodytoxin; Haemorrhagin; Antivenom; ELISA; Toxicity-neutralization potency
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Podaci o izdanju
148 (2)
2008.
178-183
objavljeno
1532-0456
10.1016/j.cbpc.2008.05.005
Povezanost rada
Temeljne medicinske znanosti, Biotehnologija, Biologija