engleski

Indirect immunofluorescence test in diagnosis of avian chlamydiosis using different conjugates

For serological detection of specific antibodies against avian chlamydiosis different tests are applicable. In Slovenia, an indirect immunofluorescence test is used for screening the immunofluorescnce status in birds. Species-specific conjugates are usually used for antibody detection. The conjugate will bind bind to specific Ig-G antibodies to form an antigen-antibody FITC complex that is visible under the immunofluorescence microscope. Commercially available conjugates are not specifically declared for use with sera from pigeons, parrots, canaries and free living birds. The aim of our study was establish the usefulness of these conjugates for other avian species (ducks, geese, pigeons, parrots, canaries, finches and free-living birds) in comparison with our home-made conjugates. Home-made conjugates were prepared in rabbits immunized with purifiedIgG from pigeons (anti pigeon conjugate). A panel of sera found to be positive with another diagnostic methods was retested and statistically evaluated. Negative sera were also included in the study. We found that commercially available conjugates declared for chicken or the other groups of birds were useful for testing parrot and pigeon sera. We got positive results with pigeon and parrot sera at the same dilution as with home-made species specific conjugates. Commercially available conjugates gave positive resilts also when canaries, finches and sparrows were tested. However, the titres of Ig-G antibodies in one of the tested conjugates were lower than when the other was used. Limited appliability of this conjugate can be expected because antibodies were confirmed only in low serum dilutions. The results show that commercially available conjugates are also useful for detection of chlamydial antibodies in other avian species, and that conjugate A gave the best results. Home-made anti-parrot and anti-pigeon conjugates are useful for testing parrots and pigeons, but not for other avian species tested, because antibodies were detected only in low serum dilutions. Anti-parrot conjugate reacted at one dilution lower compared to anti-pigeon conjugate, when pigeon sera were tested. Anti-pigeon conjugate reacted at one dilution lower compared to anti-parrot conjugate, when parrot sera were tested. Data on comparison of four different conjugates used in our study is presented in the table.

diagnosis; avian chlamydiosis

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