engleski

Serum platelet-activating factor acetylhydrolase (PAF-AH) activity and C-reactive protein (CRP) concentration in pigs after splenectomy and autologous spleen transplantation

The spleen is an important lymphatic organ with a major role in many specific and unspecific immune reactions. Autologous splenic transplantation appears to be the only possible spleen-saving technique after total splenectomy. The aim of this study was to evaluate the inflammatory response after total splenectomy and spleen autotransplantation in pigs by measuring serum platelet-activating factor acetylhydrolase activity and C-reactive protein concentration. Nineteen piglets were randomly divided into three groups: sham-operated with spleens intact (n=6), total splenectomy (n=6), and splenic autotransplantation (n=7) with small fragments of the spleen autotransplanted into the greater omentum. The blood samples were taken just before surgery and on day 1st, 5th, 12th, 26th and 40th postoperatively. PAF-AH activity and CRP concentration were assayed in the sera. PAF-AH activity was significantly increased (P<0.05) on day 5th after splenectomy and on day 26th postoperatively in sham-operated pigs while there was no significant increase after spleen autotransplantation. CRP was significantly increased after surgery in all experimental groups. Conclusion: Results demonstrate increased PAF-AH activity in pigs after splenectomy and sham-operation as well as time-course increase of CRP concentration in all groups after surgery suggesting post-injury inflammatory response due to surgical trauma.

PAF-AH; CRP; acute phase response; pigs; splenectomy; spleen autotransplantation

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