engleski

Ozone Influence on the Microbial Flora in the Root Canal

Objective: Purpose of this in vivo study was to determine the decrease in the number of root canal colonies after classic chemomechanic treatment and following additional dis-infection of the root canal with ozone. Methods: Swabs were taken on three occasions from 23 patients with visible periapical process within 5mm diameter. The paper points were inserted into transport media for anaerobs WMGA. After rubber dam placement the first swab was taken after which hand instrumentation was performed up to number 25 with much drainage with 2, 5% sodium hypochlorite and root canals were additionally instrumented with EndoEze technique. After completion of instrumentation a second swab was taken from the root canals and then they were treated with HealOzone for 40 seconds and third swab was taken. After microbial analysis, information obtained was statistically processed with SPSS computer program. Results: From the analysis of median number of aerob and anaerob bacterial colonies for all three measurements the largest decrease in the number of colonies was observed between first and second measurements for both aerobs and anaerobs. There is also a decrease between second and third but it was somewhat milder for both types of bacteria. Kruskal-Wallis test showed statistically significant difference between number of colonies of aerobes (X2=40, 483 ; df=2 ; P<0.01) and anaerobes (X2=26, 303 ; df=2 ; P<0.01). Mann-Whiney test showed a statistically significant difference between the number of colonies of both aerobe and anaerobe bacteria between the first and the third measurement (p<0.01). There was no statistically significant decrease in the number of colonies between the first and second measurement. Conclusion: After classic chemomechanic treatment of the root canal a certain amount of bacterial colonies always remains, additional disinfection of the root canal is needed for which treatment with HealOzone is very efficient.

ozone; microbial flora; root canal

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