Growth conditions in in vitro culture can induce oxidative stress in Mammillaria gracilis tissues (CROSBI ID 145016)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Balen, Biljana ; Tkalec, Mirta ; Pavoković, Dubravko ; Pevalek-Kozlina, Branka ; Krsnik-Rasol, Marijana
engleski
Growth conditions in in vitro culture can induce oxidative stress in Mammillaria gracilis tissues
In vitro propagated plants of Mammillaria gracilis Pfeiff. (Cactaceae) develop calli without any exogenous growth regulators. This habituated tissue spontaneously regenerates morphologically normal as well as hyperhydric shoots. In this study, a possible involvement of activated oxygen metabolism in habituation and hyperhydricity in in vitro propagated plants of Mammillaria gracilis Pfeiff. (Cactaceae) was investigated. A significantly higher malondialdehyde (MDA) and carbonyl contents as well as hydrogen peroxide (H2O2) production were observed in habituated callus (HC), hyperhydric regenerated shoot (HS) and tumour (TT) in comparison to normal regenerated shoot (NS). Lipoxygenase (LOX) activity showed a similar trend, with a clear increase in activity in HC and HS. The activities of antioxidative enzymes, namely peroxidase (POX), ascorbate peroxidase (APX) and catalase (CAT) were also higher in HC, HS and TT while an increase in superoxide dismutase (SOD) activity was observed in HC and HS. The majority of antioxidative isoenzymes were common to all cactus tissues, although a few tissue-specific bands were noticed. A significant decrease of phenylalanine ammonia lyase (PAL) activity, total phenolics content and lignification was found in HS, HC and TT in comparison to NS. Our results showed the appearance of a prominent oxidative stress in HC, HS and TT as well as a strong induction of the antioxidant system indicating that activated oxygen metabolism could be involved in habituation and hyperhydricity and linked to the loss of tissue organisation in M. gracilis.
Antioxidant enzymes; Habituation; Hyperhydricity; Mammillaria gracilis Pfeiff.; Oxidative stress; Tumour tissue;
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Podaci o izdanju
28 (1)
2009.
36-45
objavljeno
0721-7595
10.1007/s00344-008-9072-5