engleski

Effect of the lethal activity of 32p upon alpha amylase activity and glucose concentration in chicken blood plasma

An attempt was made to evaluate whether the activity of alpha-amylase and concentration of glucose change in blood plasma of chickens after 32P administration, and whether it helps in the diagnosis of organic or functional liver damage in the chickens caused by ionizing radiation before the appearance of clinical simptoms of radiation sickness. Fifty day old hybrid chickens of heavy Jata breeds of both sexes were treated by 32P administred i.m. in a single amount of 333 MBq per kilogram of body weight. Blood samples were taken from the wing vein on days 1, 3, 5, 7 and 10 after administration of 32P. The activity of alpha amylase was determined spectrophotometrically by using the kits produced by « ; ; ; Herbos d.d Sisak» ; ; ; (Croatia). Concentration of glucose was determined photometrically with o-toluidinom, by using also the kits produced by « ; ; ; Herbos d.d. Sisak» ; ; ; . Alpha amylase activity in the blood plasma od experimental chickens was decreased from the 3rd day after administartion of 32P to the 10th day of experiment. A statistically significant difference was recorded only on the 7th day of experiment. Concentration of glucose in the blood plasma of 32P treated chickens was signifacantlly increased on the 5th and 7th day of experiment. Obtained results indicate that alpha amylase activity and concentration of glucose are changed in the blood plasma of 32P treated chickens. It seems that only conncentration of glucose may serve as indicator of functional and/or morphological liver demage in chickens caused by ionizing radiation before the appearance of clinical symptoms of radiation sickness.

phosphorus-32 ; alpha-amylase ; glucose ; blood plasma ; chickens

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