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Comparison of 1H-31P constant-time NOESY with the 1H-31P constant-time COSY experiments for the elucidation of 1H-31P dipolar couplings in DNA-oligonucleotides and DNA-oligonucleotide/Porphyrin Complexes

The aim of the study is to confirm the binding mode and obtain detailed structural information of cationic porphyrins to DNA. For combined NMR investigations and complementary quantum mechanical calculations, model compounds have been selected to investigate the DNA-porphyrin interaction. After preliminary investigations using circular dichroism spectroscopy, indicated that the binding mode is by intercalation, 1H-31P-ct-NOESY[1] and 1H-31P-ct-COSY[2] NMR were tested to determine the 1H3´-31P Couplings in a duplex d(GCGCGC)2 and a d(GCGCGC)2/TMPyP complexes. The poster presentation will include the explanation of the used NMR pulse programs and the discussion of the obtained structural parameters. [1] Z. Wu, N. Tjandra and A. Bax, Measurement of 1H3′ -31P dipolar couplings in a DNA oligonucleotide by constant-time NOESY difference spectroscopy, Journal of Biomolecular NMR, Volume 19, Number 4, 367-370. [2] G. E. Clore, E. C. Murphy, A. M. Gronenborn, A. Bax, Determination of Three-Bond 1H3´-31P Couplings in Nucleic acids and Protein-Nucleic Acid complexes by Quantitative J Correlation Spectroscopy, Journal of Magnetic Resonance 134, 1998, 164-167.

cationic porphyrins; DNA; NMR spectroscopy; ct-NOESY; ct-COSY

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