engleski

Influence of curing mode intensities on cell culture cytotoxicity/genotoxicity

Purpose: Light curing units with different light intensity or different curing modes cause different temperature increase during composite resin polymerization. Heat generated during composite resin polymerization can cause irreversible damage of vital pulp tissue. Therefore, the aim of this study was to determine the cytotoxic/genotoxic effect of different curing modes on cell culture. Methods: A thin layer of lymphocyte cultures was cured applying three different curing modes of Bluephase C8 LED curing unit (Vivadent, Schaan, Liechtenstein, ). Cultures were exposed to light directly or through a layer (2 mm) of polymerized composite resin sample. Cells were analyzed using trypan blue exclusion test, acridine orange/ethidium bromide dying technique, and alkaline comet assay. Results: Only low intensity mode (LOP) after direct exposure significantly increased the number of nonviable lymphocytes detected using trypan blue. All curing procedures significantly increased the number of apoptotic lymphocytes regardless whether the exposure occurred directly or through the composite. LOP in direct exposure significantly elevated DNA migration compared to other curing modes. One hour after exposure significant increase in tail length and intensity for all modes and procedures was detected. However, DNA damage measured for cultures cured by LOP was higher compared to other two modes ; thus, despite of curing light intensity, longer curing time leads to greater cytotoxicity/genotoxicity in cell culture.

cytotoxicity; genotoxicity; curing modes; temperature

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