engleski

Correlation between frequency of micronuclei and status of folate and vitamin B12 in operating theatre staff

Genotoxic effects of inhalation anaesthetics have already been described. Of special concern is the exposure of medical staff in operating theatre, which is chronically exposed to low doses of different anaesthetics. In this study a group of medical workers of different professions, exposed to anaesthetics, mostly sevoflurane, isoflurane and nitrous oxide, and matched, non- exposed control group was investigated for the frequency of micronuclei (MN). The aim of this study was to investigate whether a status of folic acid (FA) and vitamin B12, as known factors of genome stability, influence a level of genome damage in studied groups. Frequency of MN elevate (p<0.05) in exposed group as compared to control (14.29 vs. 8.77). B12 concentrations were higher in control group (577.83 mg/L vs. 463.53 mg/L). Control group also had two times higher values of FA (10.15 mg/L vs. 5.89mg/L). Age, duration of exposure or smoking did not show significant influence to a MN frequency in exposed group. Further investigations will include a larger group of both exposed and control subjects to ensure more reliable results.

micronuclei frequency; operating theatre staff; folate; vitamin B12

Workshop 14: Exogenous and Endogenous Background Exposures to Carcinogens and Regulatory Consequences ; Poster Presentations S32-S89, P3: Risk Assessment ; doi:10.1016/j.toxlet.2008.06.587