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The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars (CROSBI ID 146456)

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Samardžija, Marko ; Dobranić, Tomislav ; Krušlin, Suzana ; Cergolj, Marijan ; Karadjole, Martina ; Prvanović, Nikica ; Grizelj, Juraj The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars // Veterinarski arhiv, 78 (2008), 4; 279-287

Podaci o odgovornosti

Samardžija, Marko ; Dobranić, Tomislav ; Krušlin, Suzana ; Cergolj, Marijan ; Karadjole, Martina ; Prvanović, Nikica ; Grizelj, Juraj

engleski

The use of the hypoosmotic swelling test and supravital staining in evaluation of sperm quality in boars

Semen samples from ten boars, Yorkshire and Pietrain breed, aged between two and six years were used. The sperm quality was evaluated using the following parameters: the motility (subjective), the structural supravital staining (eosine/nigrosine) and the functional integrity of sperm membrane (HOS test). The majority of HOS reactive or swelled up spermatozoa were gained in a hypoosmolar solution of 100 mOsm/kg by incubation of 30 minutes. Between middle values for HOS reactive (swollen up) spermatozoa in hypoosmolar solutions with different osmolarity, significant differences (P<0.05) were determined. Using a hypoosmotic solution with different osmolarity 100 mOsm/kg, significantly strong, positive correlations between HOS and motility (r = 0.98 ; P<0.05) were determined, between HOS test and supravital staining eosine/nigrosine (r = 0.99 ; P<0.05) and between motility and supravital staining eosine/nigrosine (r = 0.98 ; P<0.05). The results of our research have shown that the HOS test and supravital staining eosine/nigrosine could be used for routine evaluation of the functional and structural integrity of diluted boar sperm.

boar ; semen ; hypoosmotic test ; eosine/nigrosine staining

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Podaci o izdanju

78 (4)

2008.

279-287

objavljeno

0372-5480

1331-8055

Povezanost rada

Veterinarska medicina

Indeksiranost