engleski

ULTRASOUND DETECTION OF OVARIAN RESPONSE IN GOAT ASSISTED REPRODUCTION PROCEDURES

The aim of this research was to establish the importance and accuracy of the ultrasonic examination of donor and recipient goats submitted to superovulation procedures. For this reason, 17 donor goats were systematically examined by ultrasound: 1) to count the preovulatory follicles (≥ 5mm) at oestrus onset ; 2) to confirm the ovulation(s) and 3) the accuracy of the ultrasound examination was measured comparing previously findings with number of corpora lutea (CL) present when uterine flushing was performed. On the other hand, 14 recipient goat ovaries were monitored by ultrasound to assess: 1) the number of dominant follicles (≥ 5mm) present during oestrus ; 2) the ovulation and 3) the echogenic quality of CL before embryotransfer. To perform the ultrasound examination the animals were placed in dorsal recumbence and 7, 5MHz ultrasound probe was applied rectally. To predict the ovulation rate the number of large follicles (≥ 5mm) was counted by ultrasound on donor goat ovaries at the beginning of oestrus. Unovulated follicles (48h after oestrus start) were omitted. The ultrasound accuracy, measured comparing the number of ovulated follicles with number of corpora lutea counted when laparotomic flushing was performed, was 47, 5%. The ovulatory rate prediction is more accurately (72.1%) when donor goat responded with lower total number of follicles (10 or less). The response of 9 out of 14 (64%) recipient goats, after standard hormonal protocol was applied, were adequate, what means maximum 2 dominant follicles, timely occurred ovulation and good echogenic quality of respective CL. The ultrasonic examination of donor and recipient goats is appreciable tool to follow up follicular dynamics, success of superovulation procedures, confirmation of ovulation and echogenic CL quality, as well as to predict the ovulatory rate. In this way the ultrasound examinations of the ovaries could save the time and the money due to elimination of animals who did not respond to the treatment before the collection or transfer of embryos start.

ultrasonic examination; ovary; goat; superovulation; ovulation; follicle; corpus luteum; ovulatory rate

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