engleski

Oxidative stress in small-for-gestational age (SGA) term newborns and their mothers

This study used malondialdehyde (MDA) determination by HPLC and enzymatic assays for total serum peroxides and antioxidant capacity to evaluate oxidative stress in 47 healthy fullterm small-for-gestational age (SGA) newborns versus 67 appropriate-for-gestational age (AGA) newborns. Blood samples were collected at delivery from umbilical cord artery and vein and from peripheral blood of the babies on the third day after birth. Blood samples of mothers were also collected and compared with the 29 normal non-pregnant women (NPW) blood. Serum peroxide values were significantly higher in both groups of mothers than in NPW, decreasing towards the third day in AGA mothers while persisting in SGA mothers. Antioxidant capacity of sera of both groups of mothers was lower than NPW. Both SGA mothers and babies had increased MDA at delivery than AGA counterparts. MDA levels in umbilical vein were higher than in umbilical arteries, while immunohistochemistry revealed abundant presence 4-hydroxynonenal (HNE)-protein adducts only in stroma of the SGA placenta. These results show that both mothers and babies are exposed to oxidative stress during and after delivery, which is more pronounced and persistent in perinatal period of the SGA group, while lipid peroxidation in placenta could play a role in SGA pathophysiology.

oxidative stress; lipid peroxidation; malondialdehyde; 4-hydroxynonenal; peroxide; antioxidants; small-for-gestational age; appropriate-for-gestational age newborns

nije evidentirano