East Adriatic – a reservoir region of severe Citrus tristeza virus strains (CROSBI ID 149106)
Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija
Podaci o odgovornosti
Černi, Silvija ; Škorić, Dijana ; Ruščić, Jelena ; Krajačić, Mladen ; Papić, Tatjana ; Djelouah, Khaled ; Nolasco, Gustavo
engleski
East Adriatic – a reservoir region of severe Citrus tristeza virus strains
Citrus tristeza virus (CTV) represents one of the major threats for the citrus production worldwide. In the East Adriatic region, CTV symptoms are mostly absent due to the traditional citrus grafting on trifoliate orange (Poncirus trifoliata (L.) Raf.), a CTV tolerant rootstock. Therefore, the virus has been continuously spreading by the propagation of infected material. The genetic variability of CTV was studied on nineteen citrus samples, collected from orchards in the coastal region of Croatia, Montenegro and Albania, previously tested positive by ELISA and immunocapture RT-PCR. Single-strand conformation polymorphism of the amplified coat protein gene demonstrated the presence of different CTV variants in each amplicon, while sequence analysis of cloned CP gene variants confirmed their clustering into six out of the seven phylogenetic groups delineated so far. Four of these groups include sequences of severe quick decline, seedling yellows and stem pitting isolates, which were thought to be found only rarely in the Mediterranean region. Regardless of the lack of symptoms in the field, CTV isolates from the East Adriatic display high genetic variability and pathogenic potential that was additionally confirmed by biological characterization. The high percentage of mixed infections suggest their potential for further diversification and enlarge the risk of spreading severe variants into new areas.
CP gene; CTV; phylogenetic analysis; SSCP
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