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Structure of the T6 human nickel insulin derivative at 1.35 Å ; ; ; ; resolution

This paper presents results of the structural investigation on the human insulin hexamer derivative stabilised by nickel coordination. Single crystals of the Ni-insulin derivative were prepared by the hanging drop vapour diffusion crystallisation method using metal-free insulin and nickel(II) acetate tetrahydrate. The low-temperature crystal structure was determined by the single crystal X-ray crystallographic method with data extending to 1.35 Å ; ; ; ; spacing. The investigated insulin derivative exhibits the T6 form of insulin and crystallizes in the trigonal system in space group R3, with the unit cell parameters a = b = 81.41 Å ; ; ; ; and c = 33.75 Å ; ; ; ; . There are two nickel atoms per insulin hexamer and both are octahedrally coordinated by three N 2 atoms of three symmetry-related HisB10/HisD10 and three oxygen atoms of three symmetry-related water molecules.

insulin derivative; nickel; X-ray structure

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