Stress response and pathogenic potential of Campylobacter jejuni cells exposed to starvation (CROSBI ID 149514)
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Podaci o odgovornosti
Klančnik, Anja ; Guzej, Bernarda ; Jamnik, Polona ; Vučković, Darinka ; Abram, Maja ; Smole Možina, Sonja
engleski
Stress response and pathogenic potential of Campylobacter jejuni cells exposed to starvation
Campylobacter jejuni is Gram negative, fragile, spiral bacterium, worldwide known as a main cause of acute human enteritis. Like many other food borne bacteria, campylobacters must be able to survive under diverse conditions both inside the host and in the environment. Understanding the stress response mechanisms provides informations necessary to improve food processing and strategies that enhance food safety as well as clarify pathogenesis of campylobacteriosis. We investigated the relation between stress response to starvation and pathogenic potential in Campylobacter jejuni. Starvation changed morphology and physiology of C. jejuni cells. However, lower metabolic activity of 5 h starved culture was not a dormant state but probably viable but non culturable(VBNC) form of the cells, since starved C. jejuni induced heat stress resistance. The health hazard potential of starved cells is still unclear. We showed that, in spite of starvation, C. jejuni survived in vitro within Caco-2 enterocites up to 4 days, and caused systemic campylobacteriosis in vivo, in a mouse model. However, bacterial numbers in investigated organs were significantly lower and the infection was resolved sooner. Our results show that nutrient insufficiency is responsible for C. jejuni transformation, influencing but not abolishing its survival and virulence properties while in VBNC state.
Campylobacter jejuni; low nutrient conditions; bacterial sress response; virulence characteristics
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Podaci o izdanju
Povezanost rada
Temeljne medicinske znanosti