engleski

Changes of AXIN-1 and Beta-Catenin in Neuroepithelial Brain Tumors

Aims: In the present study changes of components of wnt signaling pathway were investigated in neuroepithelial brain tumors. Methods: AXIN-1 gene was tested by PCR/loss of heterozygosity (LOH). Immunostaining and Image analysis revealed the quantity and localization of relevant proteins. Results: Polymorphic marker for AXIN-1, showed LOH in 11.1% of tumors. LOH was distributed to 6.3% of glioblastomas, one in neuroepithelial dysembrioplastic tumor and one in medulloblastoma. Axin protein was observed in the cytoplasm in 68, 8% of samples, in 28, 1% in both the cytoplasm and nucleus and 3, 1% had no expression. Beta-catenin was observed in the nucleus and cytoplasm (59, 4%). In 34, 4% in cytoplasm and 6, 3% had no expression. Comparison of mean values of increase of axin and beta-catenin showed that they are significantly reversely proportional (P=0, 014). Relative quantity of beta-catenin in patients with gross deletion of AXIN1 was significantly higher in comparison to patients without LOH (P=0, 040). The highest relative value of axin was measured when the protein was in the cytoplasm and it was accompanied with the lowest quantity of beta-catenin. Conclusions: Our results demonstrate important elements of Wnt signaling in neuroepithelial brain tumors.

axin; beta-catenin; neuroepithelial brain tumors; loss of heterozygosity; Image analysis; wnt signaling

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