engleski

Kinetic Potentiometric Determination of Penicillamine and N-Acetyl-L-Cysteine Based on Reaction with Iodate

Optimization and application of simple kinetic potentiometric method for the determination of D-penicillamine (PEN) and N-acetyl-L-cysteine (NAC) in pharmaceutical is described. The method was based on the reaction between thiol and iodate, in the presence of small quantity of iodide. The changes of potentials, Δ E, in fixed time interval were measured with an iodide ion selective electrode versus a double-junction reference electrode. Good linearity between Δ E and negative logarithm of analyte concentration is achieved in the range of the studied drugs concentration from 1.0 × 10– 6 to 1.0 × 10– 4 M. The limit of detection for PEN is 9.0 × 10– 8 M and for NAC is 1.0 × 10– 7 M. The applicability of the proposed method to the determination these thiols in pharmaceutical preparation was demonstrated by investigating the effect of potential interferences and by analysis of commercial preparations.

D-penicillamine; N-acetyl-L-cysteine; kinetic potentiometry; iodide ion selective electrode

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