engleski

Comparison of postoperative pain management techniques on expression of cytolitic molecule perforin

Background and aims: The pharmacological management of postoperative pain in patients with malignancies uses very different techniques whose possible cytotoxic functions we still known very poor. The present study compared two postoperative pain management techniques in patients undergoing intravenous analgesia and patient-controlled epidural analgesia (PCEA), on expression of cytolitic molecule perforin in peripheral blood mononuclear cells of patients after colorectal carcinoma operation. Methods: Venous blood samples were taken from colon carcinoma patients and healthy volunteers and peripheral blood leukocytes (PBL) were isolated. Phenotype of lymphocytes and perforin expression were analyzed using intracellular and surface immunofluoresecncy and flow cytometric analysis (FACSCalibur). Results:Our results demonstrated that 20-30% of PBL in all examine group are perforin positive (total perforin is significantly higher than in control group, but in patient underwent epidural analgesia these expression was higher than in intravenous experimental group). Conclusions: These preliminary data suggest that human perforin is differentially expressed in distinct sub-populations of peripheral blood lymphocytes of patients with colorectal carcinoma, implicating the important role of postoperative pain management techniques on expression of perforin positive cells and their role in naï ; ve immune response and cytotoxic activity of peripheral blood lymphocytes. Acknowledgment: This work was supported by grants from the Croatian Ministry of Science (projects: 0620096-0094 and 0620096-0092)

intravenous analgesia; pain; perforin; postoperative epidural analgesia

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