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Novel Kinetic Spectrophotometric Method for Determination of Tiopronin {; ; ; ; N-(2-Mercaptopropionyl)-glycine}; ; ; ;

Novel simple kinetic spectrophotometric method for the determination of tiopronin [N-(2-mercaptopropionyl)-glycine, MPG] in pharmaceutical formulation has been developed and validated. The proposed method is based on the coupled redox-complexation reaction whose first step is reduction of Fe(III) by MPG, while second one includes the complexation of Fe(II), resulted from preceding redox reaction, with 2, 4, 6-trypyridyl-s-triazine (TPTZ). The stable Fe(TPTZ)(2)(2+) complex exhibits an absorption maximum at lambda = 593 nm. The initial rate and fixed time (at 3 min) methods were utilized for constructing the calibration graphs. The graphs were linear in concentration ranges from 1.0 x 10(-6) to 1.0 x 10(-4) mol L(-1) for both methods with limits of detection 1.3 x 10(-7) mol L(-7) and 7.5 x 10(-8) mol L(-1) for the initial rate and fixed time method, respectively. The proposed methods were successfully applied for the determination of MPG in its commercial pharmaceutical formulation.

Tiopronin ; N-(2-Mercaptopropionyl)-Glycine ; Kinetic spectrophotometry ; Initial rate method ; Fixed time method ; Pharmaceutical analysis

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