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izvor podataka: crosbi

Induction of micronuclei in human lymphocytes after occupational exposure to ultrasound (CROSBI ID 86384)

Prilog u časopisu | izvorni znanstveni rad | međunarodna recenzija

Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Besendorfer, Višnja ; Papeš, Dražena Induction of micronuclei in human lymphocytes after occupational exposure to ultrasound // Chemosphere, 38 (1999), 15; 3541-3553-x

Podaci o odgovornosti

Garaj-Vrhovac, Vera ; Kopjar, Nevenka ; Besendorfer, Višnja ; Papeš, Dražena

engleski

Induction of micronuclei in human lymphocytes after occupational exposure to ultrasound

Micronucleus assay combined with Giemsa and DAPI staining was performed on blood samples of subjects occupationally exposed ultrasound. Lymphocytes were cultivated in vitro for 72 h. At 44 h cytichalasin-B was added in cultures. Frequencies of micronuclei in exposed subjects statistically significant increased compared to control. The frequency of micronucleated cells and micronuclei in exposed subjects shows interindividual variability. Using DAPI staining we observed signal-positive and signal-negative micronuclei. Percentage of signal-positive micronuclei varies between 0 and 66.7% and signal-negative micronuclei between 33.3% and 100%. This study indicate harmful effects of ultrasound on human genome, but further investigations are necessary.

ultrasound; occupational exposure; human lymphocytes; micronuclei

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Podaci o izdanju

38 (15)

1999.

3541-3553-x

objavljeno

0045-6535

Povezanost rada

Biologija

Indeksiranost