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Placental lipid contents in type 1 diabetic pregnancy

The placenta is a complex organ which plays the central metabolic role in pregnancy. This study was designed to investigate the effect of type 1 DM on the content of lipids in deciduas free placental tissue. It was realized by comparing fatty acid contents in placentae from normal term pregnancies with those from pregnancies complicated with type-1 DM. All pregnancies were terminated by cesarean section. 72 decidua free placental semples from term pregnancies were analyzed for fatty acid content. Group I consisted of 34 normal pregnancies. Group II consisted of 38 term pregnancies with type 1 DM. The placentae were cooled and washed immediately after delivery with Lactated Ringer’ s solution at less than 4º ; ; ; C. Decidua was cut away from the cotyledons and villous tissue was obtained from multiple cotyledons. The tissue was teased of blood vessels and minced into fine pieces. Extraction of lipids was performed (Folch). Thin-layer chromatography was performed on silicic acid impregnated paper. Gas chromatography of the fatty acid methyl esters was carried on a SRI 8610C GAS CHROMATOGRAPH, („ SRI Instruments Chromatography Systems, Torrance, CA, USA). Results. Demographic data from the two groups are shown that there is no statistically significant difference between the two groups in maternal age, gestational age and birthweight. The highest amount of PL in placental tissue in both groups were found. In type 1 DM were found decrease of FFA and CE (table 1). It was found higher levels of laurinic (12:0), myristinic (14:0) and lignocerinic (24:0) fatty acids in placentae of type 1 DM in total lipids.

placenta; lipid content; diabetes mellitus type 1

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