hrvatski

Omjer sfinganina i sfinozina i koncentracija okratoksina A u urinu stanovnika s područja endemkse nefropatije

Since the mycotoxin theory of endemic nephropathy (NE) was put forward, the research on EN in Croatia and Bulgaria were focused on the nephrotoxic mycotoxin ochratoxin A (OTA) (1, 2), while the presence of other nephrotoxic mycotoxins such as fumonisin B1 (FB1) were tested in biological materials of inhabitants in EN areas only sporadically (3). It was proved that higher concentrations of OTA (4) and FB1 (5) might be found in cereals collected in EN area in Croatia than in control areas. Cereals in our country are very often contaminated with two or more mycotoxins (6). Very often finding of FB1 in our previous study and in study of Jurjević et al. indicated that the research on the simultaneous exposure to both mycotoxins would be interesting. In this study 45 human urine samples were collected in 2000 and 2005 from the same persons in the endemic village Kaniža in Croatia and 18 controls. In all samples the concentration of OTA and concentrations of sphinganine (Sa) and sphingosine (So) were measured using HPLC with fluorescence detector (7, 8). The ratio of Sa and So (Sa/So) was found to be good biomarker of exposure to FB1 in experimental animals because it increases significantly after exposure to this mycotoxin. In 2000 and 2005 the frequency of OTA- positive samples was higher in Kaniža (43% and 18 %, respectively) than in the control village (28% and 6%, respectively). The OTA concentration in samples collected in Kaniža in 2000 was higher than in 2005 (p<0.005). Although in both years Sa/So ratio was higher in Kaniža, the difference from the control group was not statistically significant. No correlation between OTA concentration and Sa/So was observed in either people from Kaniža or controls. 1. Radić et al. Toxicol Lett 1997 ; 91:105-109. 2. Petkova- Bocharova et al. IARC Scientific Publications ; 1991 ; 115:135-137. 3. Ribar et al. J Chromatogr B 2001 ; 754:511-519. 4. Puntarić et al. Croat Med J 2001 ; 42:175-180. 5. Jurjević et al. Mycotox Res 1999 ; 15:67-80. 6. Domijan et al. Food Add Contam 2005 ; 22: 677-681 7. Pascale & Visconti. Mycopathol 2000, 152, 91-95. 8. Solfrizzo et al. J. Chromatogr B 1997, 692, 87-93.

fumonisin B1 ; endemska nefropatija ; mikotoksini

nije evidentirano

engleski

Sphnganine/sphingosine ratio and ochratoxin A concentration in urine of residents from endemic nephropathy area in Croatia

Since the mycotoxin theory of endemic nephropathy (NE) was put forward, the research on EN in Croatia and Bulgaria were focused on the nephrotoxic mycotoxin ochratoxin A (OTA) (1, 2), while the presence of other nephrotoxic mycotoxins such as fumonisin B1 (FB1) were tested in biological materials of inhabitants in EN areas only sporadically (3). It was proved that higher concentrations of OTA (4) and FB1 (5) might be found in cereals collected in EN area in Croatia than in control areas. Cereals in our country are very often contaminated with two or more mycotoxins (6). Very often finding of FB1 in our previous study and in study of Jurjević et al. indicated that the research on the simultaneous exposure to both mycotoxins would be interesting. In this study 45 human urine samples were collected in 2000 and 2005 from the same persons in the endemic village Kaniža in Croatia and 18 controls. In all samples the concentration of OTA and concentrations of sphinganine (Sa) and sphingosine (So) were measured using HPLC with fluorescence detector (7, 8). The ratio of Sa and So (Sa/So) was found to be good biomarker of exposure to FB1 in experimental animals because it increases significantly after exposure to this mycotoxin. In 2000 and 2005 the frequency of OTA- positive samples was higher in Kaniža (43% and 18 %, respectively) than in the control village (28% and 6%, respectively). The OTA concentration in samples collected in Kaniža in 2000 was higher than in 2005 (p<0.005). Although in both years Sa/So ratio was higher in Kaniža, the difference from the control group was not statistically significant. No correlation between OTA concentration and Sa/So was observed in either people from Kaniža or controls. 1. Radić et al. Toxicol Lett 1997 ; 91:105-109. 2. Petkova- Bocharova et al. IARC Scientific Publications ; 1991 ; 115:135-137. 3. Ribar et al. J Chromatogr B 2001 ; 754:511-519. 4. Puntarić et al. Croat Med J 2001 ; 42:175-180. 5. Jurjević et al. Mycotox Res 1999 ; 15:67-80. 6. Domijan et al. Food Add Contam 2005 ; 22: 677-681 7. Pascale & Visconti. Mycopathol 2000, 152, 91-95. 8. Solfrizzo et al. J. Chromatogr B 1997, 692, 87-93.

fumonisin B1 ; endemic nephropathy ; mycotoxins

nije evidentirano