engleski

IL-1β-511 T/C polymorphism does not contribute to GEP-NET susceptibility

GEP-NETs represent a heterogeneous group of tumors, arising from diffuse endocrine system of gut and pancreas. Tumors are either solitary, or occur as a part of MEN-1 syndrome. The genetic basis of GEP-NETs is still largely unknown, but there is growing evidence that chronic inflammation through proinflammatory cytokines contributes to patients’ susceptibility to acquire tumors. The role of IL-1β in the gastrointestinal tract inflammation and cancerosis has been extensively studied and T allele at -511-IL-1β promotor region was associated with aggravated inflammatory reaction measured through elevated IL-1β serum levels, higher gastric cancer susceptibility and worse prognosis. The aim of our study was to estimate allelic frequency for -511 promotor SNP in IL-1ß gene in patients with GEP-NETs. DNAs obtained from 101 GEP-NET patients and 150 unrelated healthy volunteers were genotyped for the IL-1β-511 SNP using real-time PCR TaqMan® SNP genotyping assays. To compare the frequencies χ 2 test was used and results were significant if p<0.05. Although high expression genotypes (T/C and T/T) and T-allele occurred more frequently among GEP-NET patients (63.37% vs. 56.67% and 38.61% vs. 35% respectively), there were no statistically significant differences in genotypes distribution (p=0.5541), high expression genotypes (p=0.3530) or in the allelic distribution (p=0.4651) between patients and controls. Although important in pathogenesis of gastrointestinal adenocarcinoma, IL-1β seems not to contribute to GEP-NET development.

IL-1β ; polymorphisms ; GEP-NET susceptibility

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