engleski

Application of alkaline elution,Fast Micromethod and flow cytometry in detection of marine contamination

If the biomarkers of environmental quality should be accepted and implemented in monitoring programmes there is a need for them to be tested in field situations. Although the diversity data may suggest a rich populations, an undisturbed environment, the biomarker data may be indicative of a populations less able to cope with further stress. DNA damage is an inescapable aspect of life in the biosphere. The presented investigations were an attempt to examine the response of a DNA damage as a biomarker in the mussel Mytilus galloprovincialis sampled at differently contaminated areas of Istrian coast, Northern Adriatic. Measuring of DNA damage gave us information about the genotoxic risk for marine organisms exposed to mixed environmental pollution, as well as information about the presence of unknown mixture of genotoxic contaminants in the marine environment. Types of DNA damage detected are alklai-labile sites and single-strand brekas measured by Fast Micromethod, Interstrand cross-links and DNA protein cross-links by alkaline filter elution and cell cycle disturbation by flow cytometry. The applicability of all three methods for marine quality control is discussed.

Alkaline elution; Fast micromethods; Flow cytometry; DNA damage; biomonitoring; Northern Adriatic

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